Centro Investigación en Educación Superior

RECK-Mediated β1-Integrin Regulation by TGF-β1 Is Critical for Wound Contraction in Mice

CATEGORÍA(S): , , , , , , , , , .
AUTOR(ES): Jaime Gutiérrez / Cristian A. Droppelmann / Osvaldo Contreras / Chiaki Takahashi / Enrique Brandan.
Licencia Creative Commons Reconocimiento CC BY. Esta obra está bajo una Licencia Creative Commons Reconocimiento CC BY 4.0 Internacional.

Fibroblasts are critical for wound contraction; a pivotal step in wound healing. They produce and modify the extracellular matrix (ECM) required for the proper tissue remodeling. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a key regulator of ECM homeostasis and turnover. However, its role in wound contraction is presently unknown. Here we describe that Transforming growth factor type β1 (TGF-β1), one of the main profibrotic wound-healing promoting factors, decreases RECK expression in fibroblasts through the Smad and JNK dependent pathways. This TGF-β1 dependent downregulation of RECK occurs with the concomitant increase of β1-integrin, which is required for fibroblasts adhesion and wound contraction through the activation of focal adhesion kinase (FAK). Loss and gain RECK expression experiments performed in different types of fibroblasts indicate that RECK downregulation mediates TGF-β1 dependent β1-integrin expression. Also, reduced levels of RECK potentiate TGF-β1 effects over fibroblasts FAKdependent contraction, without affecting its cognate signaling. The above results were confirmed on fibroblasts derived from the Reck+/- mice compared to wild type-derived fibroblasts. We observed that Reck+/- mice heal dermal wounds more efficiently than wild type mice. Our results reveal a critical role for RECK in skin wound contraction as a key mediator in the axis: TGF-β1—RECK- β1-integrin.

Documentos relacionados

Centro Investigación en Educación Superior

Evaluación preoperatoria y predictores de morbimortalidad en resección de cáncer de pulmón

Surgical resection of lung cancer, the only available curative option today, is strongly associated with mortality. The goal during the […]

Centro Investigación en Educación Superior

In-office cup biopsy and laryngeal cytology versus operating room biopsy for the diagnosis of pharyngolaryngeal tumors: Efficacy and cost-effectiveness

Background: In-office biopsy is an effective technique to diagnose the nature of pharyngolaryngeal lesions. Methods. We selected patients with pharyngolaryngeal […]

Saltar a la barra de herramientas