Citosine-Adenine-Repeat Microsatellite of 11β-hydroxysteroid dehydrogenase 2 Gene in Hypertensive Children
|PROCEDENCIA(S):||Ciencia y Medicina, USS Santiago.|
|CATEGORÍA(S):||Biología Celular, Bioquímica, Endocrinología y Metabolismo, Química Médica, Sistema Cardiovascular y Cardíaco.|
|AUTOR(ES):||Carolina Valdivia / Cristian A. Carvajal / Carmen Campino / Fidel Allende / Alejandro Martinez Aguayo / Rene Baudrand / Andrea Vecchiola / Carlos F. Lagos / Alejandra Tapia-Castillo / Cristobal A. Fuentes / Marlene Aglony / Sandra Solari / Alexis M. Kalergis / Hernan García / Gareth I. Owen / Carlos E. Fardella.|
|TIPO DE MATERIAL:||Artículos, Investigación.|
BACKGROUND The impairment of 11β-hydroxysteroid dehydrogenase type 2 enzyme (11βHSD2) results in an inefficient conversion of cortisol to cortisone, which triggers hypertension. Cytosine-adenine repeat (CA repeat) microsatellite has been associated with low HSD11B2 gene expression. AIM To determine whether the CA-repeat length in intron 1 affect the serum cortisol to cortisone (F/E) ratio and/or blood pressure (BP) levels in pediatric subjects. SUBJECTS AND METHODS Eighty-one hypertensive (HT) and 117 normotensive (NT) subjects participated in this study. We measured BP levels, as well as the F and E and F/E ratio in morning sera and 12-hour urine samples. The length of CA repeats was determined through fragment analysis. We compared the allele distribution between the HT and NT groups, and the patients were dichotomized into groups with short alleles (S) (<21 CA repeats) or long alleles (L), and also in groups according genotype (allele combination: S/S and S/L + L/L).